Composite
Ptac-TEV-P

Part:BBa_K4225020:Design

Designed by: Leon Ritchie Salim   Group: iGEM22_HKUST   (2022-10-09)


Ptac-TEV-Pc-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1020
    Illegal NheI site found at 1043
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 855
    Illegal XhoI site found at 165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1759
    Illegal SapI.rc site found at 439
    Illegal SapI.rc site found at 787


Design Notes

Spacers are added between the basic parts and it is codon optimized for our chassis DH5α.